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plko lentiviral vector  (Addgene inc)


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    Structured Review

    Addgene inc plko lentiviral vector
    Plko Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1591 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+puro+vector/pLKO%2E1+puro+(Plasmid+%238453)/pm42863564-121-7-10
    Average 96 stars, based on 1591 article reviews
    plko lentiviral vector - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Sequencing:

    Article Title: MMEJ repair of breaks at TA repeats maintains ecDNA and cancer fitness.
    Article Snippet: .. The hairpin sequence (5′-GCGTTTCTAAACATTGCATAA-3′) was cloned into the pLKO.1-puro vector (Addgene #8453). ..

    Article Title: Modulating SPARC Expression in Mesenchymal Stem Cells Improves Secretome-Mediated Skin Regeneration and Wound Repair
    Article Snippet: .. A specific shRNA sequence targeting SPARC, with a score of 15, was selected for cloning into the pLKO.1-Puro vector (#8453, Addgene). .. The following oligonucleotide sequences were used: shSPARC Forward: 5′- CCGGGAATACATTAACGGTGCTAAACTCGAGTTTAGCACCGTTAATGTATTCTTT TTG-3′ shSPARC Reverse: 5′- AATTCAAAAAGAATACATTAACGGTGCTAAACTCGAGTTTAGCACCGTTAATGTA TTC-3′ For annealing, 1 μL of each oligonucleotide (100 μM) was mixed with 5 μL NEBuffer 2 (NEB) and Milli-Q water to 35 μL, incubated at 95°C for 3 minutes, and gradually cooled at 0.1°C/second to 16°C.

    Article Title: NOTCH4 downregulation repolarizes tumor-associated macrophages and enhances the sorafenib sensitivity of colorectal cancer cells
    Article Snippet: .. The si-NOTCH4 sequence was subsequently cloned and inserted into the PLKO.1-puro vector (#8453, Addgene, MA, USA). .. A lentiviral vector encoding the human NOTCH4 gene was constructed as an overexpression vector (pLV3-CMV-NOTCH4) (MZ59185, Shanghai Qiming Biotechnology Co., Ltd.), named pCMV-NOTCH4.

    Article Title: Hyperoside impairs mitochondrial respiration in chronic myeloid leukemia by promoting STUB1-mediated ubiquitination and degradation of NOX4.
    Article Snippet: Chronic myeloid leukemia (CML) cells exhibit a distinct reliance on oxidative phosphorylation (OXPHOS), presenting a metabolic vulnerability for therapeutic intervention.. While Hyperoside, a natural flavonol, demonstrates anti-leukemic activity, its precise molecular targets and impact on mitochondrial bioenergetics remain elusive.. In this study, we combined in silico modeling and biophysical validation to identify NADPH oxidase 4 (NOX4) as a direct molecular target of Hyperoside.

    Clone Assay:

    Article Title: MMEJ repair of breaks at TA repeats maintains ecDNA and cancer fitness.
    Article Snippet: .. The hairpin sequence (5′-GCGTTTCTAAACATTGCATAA-3′) was cloned into the pLKO.1-puro vector (Addgene #8453). ..

    Article Title: Single-cell landscape reveals PLPP2 upregulation underlying radiotherapy resistance and immune microenvironment remodeling in pancreatic cancer.
    Article Snippet: .. Generation of stable PLPP2-knockdown cell lines Murine KPC cells were transduced with lentiviral particles encoding two independent PLPP2-targeting shRNAs (shPLPP2-1, shPLPP2-2) cloned into the pLKO.1-puro vector (Addgene, #8453) or a nontargeting scramble shRNA (Sigma-Aldrich MISSION, #SHC002), followed by puromycin selection (2 μg/mL; Gibco, #A1113803) for 7 days. ..

    Article Title: Exosomal miR-484 from lung adenocarcinoma promotes osteoclastogenesis and osteolytic bone metastasis by targeting PECAM1.
    Article Snippet: .. was cloned into the pLKO.1-puro vector (Addgene, #8453), with a scrambled shRNA as negative control. ..

    Article Title: The tRNA methylation activity and specificity of NSUN2 are independent of Archease
    Article Snippet: .. To generate mESCs with stable knockdown of archease (Zbtb80s), two complementary DNA oligonucleotides encoding a short hairpin RNA (shRNA) targeting archease (Supplementary Table 1) were hybridized and cloned into the AgeI/EcoRI sites of the pLKO.1-puro vector (Addgene #8453). .. Lentiviral particles were produced in HEK293T cells by co-transfecting the recombinant pLKO.1 vector with the psPAX2 packaging plasmid and the pMD2.G envelope plasmid, following the Addgene pLKO.1 protocol (https://www.addgene.org/protocols/plko/).

    Article Title: NOTCH4 downregulation repolarizes tumor-associated macrophages and enhances the sorafenib sensitivity of colorectal cancer cells
    Article Snippet: .. The si-NOTCH4 sequence was subsequently cloned and inserted into the PLKO.1-puro vector (#8453, Addgene, MA, USA). .. A lentiviral vector encoding the human NOTCH4 gene was constructed as an overexpression vector (pLV3-CMV-NOTCH4) (MZ59185, Shanghai Qiming Biotechnology Co., Ltd.), named pCMV-NOTCH4.

    Plasmid Preparation:

    Article Title: MMEJ repair of breaks at TA repeats maintains ecDNA and cancer fitness.
    Article Snippet: .. The hairpin sequence (5′-GCGTTTCTAAACATTGCATAA-3′) was cloned into the pLKO.1-puro vector (Addgene #8453). ..

    Article Title: Exosomal miR-484 from lung adenocarcinoma promotes osteoclastogenesis and osteolytic bone metastasis by targeting PECAM1.
    Article Snippet: .. was cloned into the pLKO.1-puro vector (Addgene, #8453), with a scrambled shRNA as negative control. ..

    Article Title: Modulating SPARC Expression in Mesenchymal Stem Cells Improves Secretome-Mediated Skin Regeneration and Wound Repair
    Article Snippet: .. A specific shRNA sequence targeting SPARC, with a score of 15, was selected for cloning into the pLKO.1-Puro vector (#8453, Addgene). .. The following oligonucleotide sequences were used: shSPARC Forward: 5′- CCGGGAATACATTAACGGTGCTAAACTCGAGTTTAGCACCGTTAATGTATTCTTT TTG-3′ shSPARC Reverse: 5′- AATTCAAAAAGAATACATTAACGGTGCTAAACTCGAGTTTAGCACCGTTAATGTA TTC-3′ For annealing, 1 μL of each oligonucleotide (100 μM) was mixed with 5 μL NEBuffer 2 (NEB) and Milli-Q water to 35 μL, incubated at 95°C for 3 minutes, and gradually cooled at 0.1°C/second to 16°C.

    Article Title: NCKAP1 Is Essential for SLC7A11-Dependent Disulfide Stress-Induced Cell Death Under Glucose Starvation in an Oral Squamous Cell Model
    Article Snippet: .. Stable NCKAP1 knockdown was generated using pLKO.1-puro vector (Addgene, Watertown, MA, USA, #8453) carrying either shRNA sequences targeting NCKAP1 (sh-NCKAP1, 5’- CCGG AACATCTACAAATGTGAAGTACTCGAG CTTCACATTTGTAGATGTTATTTTTT-3’) or the scrambled (sh-NC, 5’-CCGGCAACAAGATGAAGAGCACC AACTCGAGTTGGTGCTCTTCATCTTGTTGTTTTT -3’). .. HEK293T packaging cells (CL-0005, Procell Life Science & Technology, Wuhan, China) were maintained in high-glucose DMEM with 10% FBS and 1% P/S.

    Article Title: The tRNA methylation activity and specificity of NSUN2 are independent of Archease
    Article Snippet: .. To generate mESCs with stable knockdown of archease (Zbtb80s), two complementary DNA oligonucleotides encoding a short hairpin RNA (shRNA) targeting archease (Supplementary Table 1) were hybridized and cloned into the AgeI/EcoRI sites of the pLKO.1-puro vector (Addgene #8453). .. Lentiviral particles were produced in HEK293T cells by co-transfecting the recombinant pLKO.1 vector with the psPAX2 packaging plasmid and the pMD2.G envelope plasmid, following the Addgene pLKO.1 protocol (https://www.addgene.org/protocols/plko/).

    Article Title: NOTCH4 downregulation repolarizes tumor-associated macrophages and enhances the sorafenib sensitivity of colorectal cancer cells
    Article Snippet: .. The si-NOTCH4 sequence was subsequently cloned and inserted into the PLKO.1-puro vector (#8453, Addgene, MA, USA). .. A lentiviral vector encoding the human NOTCH4 gene was constructed as an overexpression vector (pLV3-CMV-NOTCH4) (MZ59185, Shanghai Qiming Biotechnology Co., Ltd.), named pCMV-NOTCH4.

    Article Title: Hyperoside impairs mitochondrial respiration in chronic myeloid leukemia by promoting STUB1-mediated ubiquitination and degradation of NOX4.
    Article Snippet: Chronic myeloid leukemia (CML) cells exhibit a distinct reliance on oxidative phosphorylation (OXPHOS), presenting a metabolic vulnerability for therapeutic intervention.. While Hyperoside, a natural flavonol, demonstrates anti-leukemic activity, its precise molecular targets and impact on mitochondrial bioenergetics remain elusive.. In this study, we combined in silico modeling and biophysical validation to identify NADPH oxidase 4 (NOX4) as a direct molecular target of Hyperoside.

    Transduction:

    Article Title: Single-cell landscape reveals PLPP2 upregulation underlying radiotherapy resistance and immune microenvironment remodeling in pancreatic cancer.
    Article Snippet: .. Generation of stable PLPP2-knockdown cell lines Murine KPC cells were transduced with lentiviral particles encoding two independent PLPP2-targeting shRNAs (shPLPP2-1, shPLPP2-2) cloned into the pLKO.1-puro vector (Addgene, #8453) or a nontargeting scramble shRNA (Sigma-Aldrich MISSION, #SHC002), followed by puromycin selection (2 μg/mL; Gibco, #A1113803) for 7 days. ..

    shRNA:

    Article Title: Single-cell landscape reveals PLPP2 upregulation underlying radiotherapy resistance and immune microenvironment remodeling in pancreatic cancer.
    Article Snippet: .. Generation of stable PLPP2-knockdown cell lines Murine KPC cells were transduced with lentiviral particles encoding two independent PLPP2-targeting shRNAs (shPLPP2-1, shPLPP2-2) cloned into the pLKO.1-puro vector (Addgene, #8453) or a nontargeting scramble shRNA (Sigma-Aldrich MISSION, #SHC002), followed by puromycin selection (2 μg/mL; Gibco, #A1113803) for 7 days. ..

    Article Title: Exosomal miR-484 from lung adenocarcinoma promotes osteoclastogenesis and osteolytic bone metastasis by targeting PECAM1.
    Article Snippet: .. was cloned into the pLKO.1-puro vector (Addgene, #8453), with a scrambled shRNA as negative control. ..

    Article Title: Modulating SPARC Expression in Mesenchymal Stem Cells Improves Secretome-Mediated Skin Regeneration and Wound Repair
    Article Snippet: .. A specific shRNA sequence targeting SPARC, with a score of 15, was selected for cloning into the pLKO.1-Puro vector (#8453, Addgene). .. The following oligonucleotide sequences were used: shSPARC Forward: 5′- CCGGGAATACATTAACGGTGCTAAACTCGAGTTTAGCACCGTTAATGTATTCTTT TTG-3′ shSPARC Reverse: 5′- AATTCAAAAAGAATACATTAACGGTGCTAAACTCGAGTTTAGCACCGTTAATGTA TTC-3′ For annealing, 1 μL of each oligonucleotide (100 μM) was mixed with 5 μL NEBuffer 2 (NEB) and Milli-Q water to 35 μL, incubated at 95°C for 3 minutes, and gradually cooled at 0.1°C/second to 16°C.

    Article Title: NCKAP1 Is Essential for SLC7A11-Dependent Disulfide Stress-Induced Cell Death Under Glucose Starvation in an Oral Squamous Cell Model
    Article Snippet: .. Stable NCKAP1 knockdown was generated using pLKO.1-puro vector (Addgene, Watertown, MA, USA, #8453) carrying either shRNA sequences targeting NCKAP1 (sh-NCKAP1, 5’- CCGG AACATCTACAAATGTGAAGTACTCGAG CTTCACATTTGTAGATGTTATTTTTT-3’) or the scrambled (sh-NC, 5’-CCGGCAACAAGATGAAGAGCACC AACTCGAGTTGGTGCTCTTCATCTTGTTGTTTTT -3’). .. HEK293T packaging cells (CL-0005, Procell Life Science & Technology, Wuhan, China) were maintained in high-glucose DMEM with 10% FBS and 1% P/S.

    Article Title: The tRNA methylation activity and specificity of NSUN2 are independent of Archease
    Article Snippet: .. To generate mESCs with stable knockdown of archease (Zbtb80s), two complementary DNA oligonucleotides encoding a short hairpin RNA (shRNA) targeting archease (Supplementary Table 1) were hybridized and cloned into the AgeI/EcoRI sites of the pLKO.1-puro vector (Addgene #8453). .. Lentiviral particles were produced in HEK293T cells by co-transfecting the recombinant pLKO.1 vector with the psPAX2 packaging plasmid and the pMD2.G envelope plasmid, following the Addgene pLKO.1 protocol (https://www.addgene.org/protocols/plko/).

    Article Title: Hyperoside impairs mitochondrial respiration in chronic myeloid leukemia by promoting STUB1-mediated ubiquitination and degradation of NOX4.
    Article Snippet: Chronic myeloid leukemia (CML) cells exhibit a distinct reliance on oxidative phosphorylation (OXPHOS), presenting a metabolic vulnerability for therapeutic intervention.. While Hyperoside, a natural flavonol, demonstrates anti-leukemic activity, its precise molecular targets and impact on mitochondrial bioenergetics remain elusive.. In this study, we combined in silico modeling and biophysical validation to identify NADPH oxidase 4 (NOX4) as a direct molecular target of Hyperoside.

    Selection:

    Article Title: Single-cell landscape reveals PLPP2 upregulation underlying radiotherapy resistance and immune microenvironment remodeling in pancreatic cancer.
    Article Snippet: .. Generation of stable PLPP2-knockdown cell lines Murine KPC cells were transduced with lentiviral particles encoding two independent PLPP2-targeting shRNAs (shPLPP2-1, shPLPP2-2) cloned into the pLKO.1-puro vector (Addgene, #8453) or a nontargeting scramble shRNA (Sigma-Aldrich MISSION, #SHC002), followed by puromycin selection (2 μg/mL; Gibco, #A1113803) for 7 days. ..

    Negative Control:

    Article Title: Exosomal miR-484 from lung adenocarcinoma promotes osteoclastogenesis and osteolytic bone metastasis by targeting PECAM1.
    Article Snippet: .. was cloned into the pLKO.1-puro vector (Addgene, #8453), with a scrambled shRNA as negative control. ..

    Cloning:

    Article Title: Modulating SPARC Expression in Mesenchymal Stem Cells Improves Secretome-Mediated Skin Regeneration and Wound Repair
    Article Snippet: .. A specific shRNA sequence targeting SPARC, with a score of 15, was selected for cloning into the pLKO.1-Puro vector (#8453, Addgene). .. The following oligonucleotide sequences were used: shSPARC Forward: 5′- CCGGGAATACATTAACGGTGCTAAACTCGAGTTTAGCACCGTTAATGTATTCTTT TTG-3′ shSPARC Reverse: 5′- AATTCAAAAAGAATACATTAACGGTGCTAAACTCGAGTTTAGCACCGTTAATGTA TTC-3′ For annealing, 1 μL of each oligonucleotide (100 μM) was mixed with 5 μL NEBuffer 2 (NEB) and Milli-Q water to 35 μL, incubated at 95°C for 3 minutes, and gradually cooled at 0.1°C/second to 16°C.

    Knockdown:

    Article Title: NCKAP1 Is Essential for SLC7A11-Dependent Disulfide Stress-Induced Cell Death Under Glucose Starvation in an Oral Squamous Cell Model
    Article Snippet: .. Stable NCKAP1 knockdown was generated using pLKO.1-puro vector (Addgene, Watertown, MA, USA, #8453) carrying either shRNA sequences targeting NCKAP1 (sh-NCKAP1, 5’- CCGG AACATCTACAAATGTGAAGTACTCGAG CTTCACATTTGTAGATGTTATTTTTT-3’) or the scrambled (sh-NC, 5’-CCGGCAACAAGATGAAGAGCACC AACTCGAGTTGGTGCTCTTCATCTTGTTGTTTTT -3’). .. HEK293T packaging cells (CL-0005, Procell Life Science & Technology, Wuhan, China) were maintained in high-glucose DMEM with 10% FBS and 1% P/S.

    Article Title: The tRNA methylation activity and specificity of NSUN2 are independent of Archease
    Article Snippet: .. To generate mESCs with stable knockdown of archease (Zbtb80s), two complementary DNA oligonucleotides encoding a short hairpin RNA (shRNA) targeting archease (Supplementary Table 1) were hybridized and cloned into the AgeI/EcoRI sites of the pLKO.1-puro vector (Addgene #8453). .. Lentiviral particles were produced in HEK293T cells by co-transfecting the recombinant pLKO.1 vector with the psPAX2 packaging plasmid and the pMD2.G envelope plasmid, following the Addgene pLKO.1 protocol (https://www.addgene.org/protocols/plko/).

    Article Title: Hyperoside impairs mitochondrial respiration in chronic myeloid leukemia by promoting STUB1-mediated ubiquitination and degradation of NOX4.
    Article Snippet: Chronic myeloid leukemia (CML) cells exhibit a distinct reliance on oxidative phosphorylation (OXPHOS), presenting a metabolic vulnerability for therapeutic intervention.. While Hyperoside, a natural flavonol, demonstrates anti-leukemic activity, its precise molecular targets and impact on mitochondrial bioenergetics remain elusive.. In this study, we combined in silico modeling and biophysical validation to identify NADPH oxidase 4 (NOX4) as a direct molecular target of Hyperoside.

    Generated:

    Article Title: NCKAP1 Is Essential for SLC7A11-Dependent Disulfide Stress-Induced Cell Death Under Glucose Starvation in an Oral Squamous Cell Model
    Article Snippet: .. Stable NCKAP1 knockdown was generated using pLKO.1-puro vector (Addgene, Watertown, MA, USA, #8453) carrying either shRNA sequences targeting NCKAP1 (sh-NCKAP1, 5’- CCGG AACATCTACAAATGTGAAGTACTCGAG CTTCACATTTGTAGATGTTATTTTTT-3’) or the scrambled (sh-NC, 5’-CCGGCAACAAGATGAAGAGCACC AACTCGAGTTGGTGCTCTTCATCTTGTTGTTTTT -3’). .. HEK293T packaging cells (CL-0005, Procell Life Science & Technology, Wuhan, China) were maintained in high-glucose DMEM with 10% FBS and 1% P/S.

    Control:

    Article Title: Hyperoside impairs mitochondrial respiration in chronic myeloid leukemia by promoting STUB1-mediated ubiquitination and degradation of NOX4.
    Article Snippet: Chronic myeloid leukemia (CML) cells exhibit a distinct reliance on oxidative phosphorylation (OXPHOS), presenting a metabolic vulnerability for therapeutic intervention.. While Hyperoside, a natural flavonol, demonstrates anti-leukemic activity, its precise molecular targets and impact on mitochondrial bioenergetics remain elusive.. In this study, we combined in silico modeling and biophysical validation to identify NADPH oxidase 4 (NOX4) as a direct molecular target of Hyperoside.



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